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Vol. 4 Issue 1 June 2013
Research Article
Acute toxicity of copper sulphate to African catfish, (Clarias gariepinus)
Adil A. Wani, M. Sikdar-Bar and Hilal Ahmad Khan
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Research Article


GERF Bulletin of Biosciences

June 2013, 4(1):14-18


Acute toxicity of copper sulphate to African catfish, (Clarias gariepinus)


Adil A. Wani*, M. Sikdar-Bar and Hilal Ahmad Khan


Department of Zoology, Dr. Harisingh Gour University, Sagar (M.P), India


*Corresponding author:




In this study, 96 hrs LC50 values of copper sulphate (CuSO4.5H2O), a highly toxicant heavy metal, on African catfish, Clarias gariepinus of average weight 98.43 ± 24.09 g and length 20.5 ± 2.5 cm was determined. The acute toxicity tests were performed according to the static non-renewable bioassay procedure. The experimental design consisted of a control and six concentrations (24, 31, 38, 44, 50 and 55 ppm) of copper sulphate, with two replicates per group and twenty fishes in each replicate. The 96 hour LC50 value based on probit analysis was found to be 40.86 ppm; the lower and upper lethal confidence limit for copper sulphate indicated a wide range between 37.47 to 44.58 ppm. Susceptibility of catfish, Clarias gariepinus to lethal effect of copper sulphate was found to be duration and concentration dependent as mortality was increased with an increase in its concentration. Results indicated that copper sulphate is toxic to fish, to moderate extent even at lower concentrations. Therefore, the present investigation may provide useful guidance that can be exploited by the aquaculturists to formulate the safety levels of copper sulphate in water bodies.


© 2013 Wani et al.; licensee Green Earth Research Foundation. This article distributed under terms of Creative Commons Attribution License (

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