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Vol. 1 Issue 1 December 2010
Short Communication
Determination of LC50 of cadmium chloride in Heteropneustes fossilis
Anu Singh, D.K. Jain and Puneet Kumar
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Short communication


GERF Bulletin of Biosciences

December 2010, 1(1): 21-24


Determination of LC50 of cadmium chloride in Heteropneustes fossilis


Anu Singh1, D.K. Jain2* and Puneet Kumar3


1Laboratory of Immunocytochemistry, Bareilly College, Bareilly-243006 (U.P.), India

2Department of Zoology, Bareilly College, Bareilly, Bareilly – 243

3Indian Veterinary Research Institute, Izatnagar – 243122, Bareilly (UP), India


*Corresponding author:




The aim of present study was to determine the LC50 value of cadmium chloride in Heteropneustes fossilis. Experimental fishes were procured from fish markets/fish ponds, which were measured an average length 16±4 cm and weight 28±5 g. Thereafter they were kept in sterile aquaria and fed with fish meal @ of 4% body weight of fish. Seven groups of experimental fish (containing 10 fish in each group) were exposed to different concentrations of cadmium chloride i.e. 0, 30, 40, 50, 60, 70 and 80ppm respectively for 96 h. Simultaneously they were fed with dried fish meal. Physicochemical parameters like D.O., Hardness, Chlorine etc. of aquaria water and the mortality rate of fish were monitored daily. It was found that the 50% lethal concentration (LC50) of cadmium chloride for Heteropneustes fossilis is 50.41 ppm and LC100 (95% confidence limit) 86.32 ppm.


© 2010 Singh et al.; licensee Green Earth Research Foundation. This article distributed under terms of Creative Commons Attribution License (

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