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Manuscript Details
Vol. 7 Issue 2 December 2016
Research Article
Histopathologic manifestations of wistar rats exposed to virgin engine oil
A A Iyanda, C I Iheakanwa and O Aina O O
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Research Article


GERF Bulletin of Biosciences

December 2016, 7(2):1-5

Published Online: 30 December 2016


Histopathologic manifestations of wistar rats exposed to virgin engine oil


A A Iyanda1*, C I Iheakanwa2 and O Aina O O2


*1Department of Chemical Pathology, College of Health Sciences, Ladoke Akintola University of Technology, Osogbo, Nigeria

2Department of Veterinary Anatomy, University of Ibadan, Ibadan, Nigeria



*Corresponding author:




Constant contact with used-engine oil has been identified as a cause of many biochemical derailments which eventually results in histopathological changes, yet only slight differences in chemical composition exists between used and virgin engine oil. In parts of Africa, virgin engine oil is widely applied by human subjects for both cosmetics and therapeutically purposes and hence, the present study was undertaken to evaluate the repeated daily exposure to virgin engine oil and its capability of causing tissue damage. Two groups of albino rats consisting of six rats per group were used for the study. The first group was administered with 1.0 ml/kg body weight of virgin engine oil through the oral route as contaminant of feed while the second group served as the control. The study was for a period of 30 days after which the rats were sacrificed and organs harvested. Sections of investigated tissues were fixed in 10% neutral buffered formalin. Haematoxylin and Eosin staining technique was used for histopathological study. Results of the study revealed that exposure to engine oil caused significant histological alterations to cells of

liver, kidney, lung and intestine. Results of the study suggest that incessant exposure to virgin engine oil can lead to tissue damage.


Key Words: Virgin engine oil, oral route, dermal route, histopathological changes.


 © Lynda et al., 2016; licensee Green Earth Research Foundation. This article distributed under terms of Creative Commons Attribution License ( Which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.

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